EFB (European Foulbrood)

Summary

The European Foulbrood (EFB) is a bacterial disease that affects the honey bee brood. The genetic resistance of some species of bees to this disease may allow, especially in favourable environmental conditions, to overcome the infection without suffering serious damage. However, it should be noted that, even if characterised by a better prognosis than the American Foulbrood, in some areas the EFB has a more malignant manifestation, seriously damaging even very strong bee colonies.

This practice describes the causes and symptoms of EFB, how the disease spreads, and how to address and prevent the disease.

Description

CAUSES

EFB is caused by the streptococcus bacterium Melissococcus pluton (M. pluton), often associated with other bacterial agents, including: Bacillus alvei, Streptococcus faecalis, Achromobacter eurydice, Paenibacillus alvei and Bacillus laterosporus. Depending on the type of bacteria associated with the bacterium M. pluton, the EFB can occur with different symptoms (e.g. the presence / absence of an unpleasant acid smell).

M. pluton is a germ that is quite resistant to adverse environmental conditions (e.g. it remains viable for several months in pollen).

TRANSMISSION

The bacterium develops inside the hive at the brood level. This disease spreads orally inside the hive by the nurse bees that, in an attempt to clean up the cells by the dead larvae, get contaminated with the spores and they transmit them to the brood when they go to feed it.

The disease can be spread from hive to hive or apiary to apiary by the bees (especially when bees rob a diseased hive) and by the beekeeper (with the use of infected honey to feed healthy colonies, moving diseased colonies during migratory beekeeping, trade of infected tools, use of contaminated equipment, moving of combs from one hive to another, etc.).

The disease, while being able to occur throughout the year, is more common in spring when there is more brood. The bacterium can spread through honey with infected combs (through pollen, honey, brood, etc.).

 

The development of EFB could be favoured by an imbalance between the number of larvae and that of the nurse bees. In addition, the EFB would seem to be more common in cold and rainy springs, where there may be food shortages, particularly of protein for the brood (lack of pollen). It has also been observed that the quality and quantity of the sources of nectar and pollen are able to influence the course of the disease.

The health status of the colony is very important for the development of the disease inside the colony: weak colonies or colonies that are stressed for any reason (food shortage, migratory beekeeping, pesticides, etc.), as well as genetically more sensitive colonies, are especially prone to this disease. 

Healthy and strong colonies will be able to recover from the disease by themselves if the season guarantees adequate food sources (pollen, nectars and flowers).

SYMPTOMS

The transmission of EFB from the adult bee to the larva takes place orally. After the infection, the larvae die in a few days (regardless of whether the larvae are working bees, drones or queens). Unlike the American Foulbrood, M. pluton kills the larvae before the cells’ capping.

The death of the larvae occurs with open cells and this is one of the features that allows to differentiate the EFB from the AFB (Fig. 1). Only in the case of serious infection with EFB, the larvae can die in capped cells.

Fig. 1: Larval death with open cells

Fig. 1: Larval death with open cells

Another important feature useful to recognise this disease, is that the affected larvae instead of being horizontally positioned on one side in a C-shape, adhering to the back of the cells, they often change position.

The infected larvae initially lose their pearly white colour to become first opaque, then yellowish and finally yellowish-brown (Fig. 2). After death, the larva becomes darker and decomposes, turning into a soft brown mass which is neither viscous nor stringy, unlike the larvae infested with AFB (Fig. 3).

This mass dries up forming a dark rust flake similar to that of AFB but, unlike the latter, it is easily removable from the cell.

Fig. 2:  The infected larvae initially lose their pearly white colour to become first opaque, then yellowish and finally yellowish-brown

Fig. 2: The infected larvae initially lose their pearly white colour to become first opaque, then yellowish and finally yellowish-brown

Fig. 3: After death, the larva becomes darker and decomposes, turning into a soft brown mass which is neither viscous nor stringy

Fig. 3: After death, the larva becomes darker and decomposes, turning into a soft brown mass which is neither viscous nor stringy

The brood appears scattered, with cells containing yellowed dead larvae. Depending on the bacteria present the dead larvae may give off smells of different intensity.

The Melissococcus pluton leads to a sour smell, with flabby, but intact larvae; if, however, it is associated with Bacillus alvei, the brood has a putrid smell with molten larvae (but not ropey as with AFB). There are also intermediate forms in which the combs do not give off any smell.

When the disease is not well developed, especially if you replace the queen and you are in a favourable time of the year (with the presence of abundant flowers providing nectar and pollen), bees may be able to clean up all the affected cells and the disease can disappear  spontaneously, thus preventing the infection from spreading to the rest of the apiary.

Main differences between European and American foulbrood

DIAGNOSIS

For the field diagnosis it is sufficient to examine the brood and to look for the symptoms described above (scattered brood, yellowed dead larvae in open cells, sour smell, etc.), which can be combined with the use of a rapid diagnostic kit, easily available on the market (Fig. 4). For disease confirmation, you can send a sample of the dead larvae to specialised laboratories where the pathogens responsible for the disease can be isolated (Fig.5). 

Fig.4: Rapid diagnostic kit (positive result above, negative below)

Fig.4: Rapid diagnostic kit (positive result above, negative below)

Fig. 5: Sample of dead larvae taken to be sent to specialised laboratories for the diagnosis

Fig. 5: Sample of dead larvae taken to be sent to specialised laboratories for the diagnosis

CONTROL

Take the appropriate actions as soon as possible to control the infection, such as:

  • destruction by incineration of the infected colonies (honeycombs and honey bees; the hives, if in good state, could be disinfected). This action is suggested in case of advanced stage of the disease, weak colonies or low prevalence of the disease in the apiary;
  • accurate disinfection of all objects used for the manipulation of infected hives, including equipment used for operations by the beekeeper (e.g. the hive tool, the gloves, the suit, the honey extractor, etc.);
  • shook swarm method, consisting in shaking the hives from the infected combs into a clean hive with new foundation.

PREVENTION

  • Ensure that colonies have always available stocks of food (pollen and honey).
  • Do not use honeys suspected of being infected to feed the bees.
  • Do not move combs from a hive to another without checking their healthy conditions.
  • Renew the combs every 2-3 years (about 30% of the combs per year).
  • Remove the queen from the infected colonies.

See related technologies published on TECA by Apimondia and IZSLT on bee diseases:

  1. Good beekeeping practices
  2. Main diseases of honey bees
  3. Nosemosis
  4. Varroa mites (Varroatosis or Varroosis)
  5. AFB (American Foulbrood)
  6. EFB (European foulbrood)
  7. Bee viruses

 

Authors:

Formato G. (Istituto Zooprofilattico Sperimentale del Lazio e della Toscana - Italy), Menegotto A. (Conservation Global - South Africa), Jannoni-Sebastianini R. (Apimondia - International Federation of Beekeepers’ Associations - Italy), EFB (European Foulbrood), 2015

Further reading

Ferrari C., La Peste Europea. In “Aspetti igienico-sanitari in apicoltura” published by Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “M. Aleandri”, Italy, August 2007, 19-21

Ellis J., Honey Bee Research and Extension Lab at the University of Florida, Video Field Guide to Beekeeping – American & European Foulbrood, January 2012, https://www.youtube.com/watch?v=s74WIPpGRHs

Created date

Wed, 17/06/2015 - 12:15

Source(s)

Apimondia

Apimondia, the International Federation of Beekeepers’ Associations, is the world organisation representing the interests of apiculture and aims to facilitate links between beekeepers, scientists and all involved with apiculture. Apimondia stems from the International Committee of Apicultural Congresses created in 1893 holding the first congress in 1897 in Belgium. Apimondia in its current institutional form was founded in the Netherlands in 1949 and its core business is the organisation of international apicultural congresses and symposia. Today Apimondia work remains truly international: Apimondia is run on a basis of co-operation between beekeepers and scientists from many countries as well as international organisations. A special feature of Apimondia is the wide range of working languages used in publications and at meetings.

You can also visit Apimondia linked websites to find out more:

www.apimondia.org

www.apimondia2013.com

www.apimondia2015.com

www.beethecampaign.org

 

 

Apimondia, la Federación Internacional de las Asociaciones de Apicultores, es la organización mundial que representa los intereses de la apicultura y su objetivo es facilitar los vínculos entre los apicultores, los científicos y todos los involucrados con la apicultura. Apimondia deriva del Comité Internacional de los Congresos Apícolas creado en 1893 y con la celebración del primer congreso en 1897 en Bélgica.  Apimondia, en su forma institucional actual, fue fundada en los Países Bajos en 1949 y su actividad principal es la organización de congresos y simposios apícolas internacionales. Hoy el trabajo de Apimondia permanece verdaderamente internacional: Apimondia realiza su programa sobre la base de la cooperación entre los apicultores y los científicos de muchos países, así como organizaciones internacionales. Una característica especial de Apimondia es la amplia gama de idiomas utilizados en las publicaciones y en las reuniones de trabajo.

Para más informes puede visitar los sitios web de Apimondia:

www.apimondia.org

www.apimondia2013.com

www.apimondia2015.com

www.beethecampaign.org

Contacts: 
Contact person: 
Riccardo Jannoni-Sebastianini
Contact email: 
Contact person: 
Apimondia Secretary
Contact email: 
Country: 
Italy

IZSLT - Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri”

The Istituto Zooprofilattico Sperimentale del Lazio e della Toscana “Mariano Aleandri” (IZSLT) is a public body operating in the frame of the National Health Service with duties related to animal health and welfare and food safety. In such areas it provides services in the diagnosis of animal diseases and zoonoses, microbiological, chemical and physical controls over safety of foods, food production chains and animal feed. In the same areas it performs research, epidemiological surveillance, continuous training and international cooperation activities.

IZSLT's mission is to ensure that animal health and welfare, hygiene of farms, primary productions, safety of foods and animal feed comply with the relevant legislation.

To effectively fulfill its mission, the Institute carries out:

  • diagnostic service over animal diseases and zoonoses;
  • scientific and technical support to veterinary and public health services for controls on animals, food and feed;
  • laboratory tests to verify the health status of animals;
  • technical and scientific support to monitor veterinary medicines;
  • research on animal health and welfare, food safety and hygiene of farming and livestock products;
  • studies on animal welfare and development of alternatives to the use of animals in experiments;
  • studies to monitor the safety of food of animal origin and feed;
  • scientific and technological cooperation with other research institutes;
  • epidemiological surveillance on animal health and food safety, on livestock products and on environmental factors affecting the above;
  • studies on the health risks for humans linked to animals and animal products;
  • support, technical assistance and hygiene information to manufacturers of food of animal origin;
  • production of vaccines and laboratory diagnostics for the improvement of animal health.

In the frame of IZSLT activities, the Apiculture Unit’s mission (http://www.izslt.it/apicoltura/) is to certify and guarantee the health and welfare of bees, and the hygiene and safety of the hive products, through: diagnosis of bee diseases and analyses on hive products, support to Governmental Institutions in drawing up legislation, research activity, collaboration with other laboratories or institutions, technical support and training for operators, pollution environmental monitoring using honey bees and protection of honey bee biodiversity.

Contacts: 
Contact person: 
Giovanni Formato
Country: 
Italy